Rhof^tm1.2Tac
Targeted Allele Detail

Summary

• Symbol: Rhof^tm1.2Tac
• Name: ras homolog family member F (in filopodia); targeted mutation 1.2, Taconic
• MGI ID: MGI:5496692
• Gene: Rhof Location: Chr5:123256243-123270692 bp, - strand Genetic Position: Chr5, 62.77 cM
• Alliance: Rhof^tm1.2Tac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195926
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 3. An Additional loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neomycin resistance cassette and left exons 3 and 4 floxed. Cre-mediated recombination removed exons 3 and 4. Western blot analysis confirmed the absence of protein expression in platelets. (J:195926)

Generation of the Rhof^tm1.2Tac allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhof Mutation: 18 strains or lines available

References

• Original: J:195926 Goggs R, et al., The small GTPase Rif is dispensable for platelet filopodia generation in mice. PLoS One. 2013;8(1):e54663
• All: 1 reference(s)