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Gt(ROSA)26Sortm1.1(Aco1*)Mwh
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1.1(Aco1*)Mwh
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Matthias W Hentze
MGI ID: MGI:5496785
Synonyms: IRP1*
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1.1(Aco1*)Mwh page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:196374
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence)
Mutation:    Insertion
 
Gt(ROSA)26Sortm1.1(Aco1*)Mwh expresses 1 gene
 
Mutation detailsA cDNA encoding Aco1 (Irp1) was mutated by PCR so that 3 cysteines (C437, C503 and C506) were changed to serines and another cysteine (C118) to alanine. These mutations putatively stabilize the apoprotein in the presence of heme. The mutated cDNA (Aco1*) was fused to a FLAG tag and inserted into a plasmid behind a loxP-flanked STOP sequence. The final construct was electroporated into E14 ES cells for homologous recombination. Correctly targeted ES cells were used to generate mice carrying the conditional allele. These animals were crossed to Hprttm1(cre)Mnn mice to delete the STOP cassette. (J:196374)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  992 strains or lines available
References
Original:  J:196374 Casarrubea D, et al., Abnormal body iron distribution and erythropoiesis in a novel mouse model with inducible gain of iron regulatory protein (IRP)-1 function. J Mol Med (Berl). 2013 Jul;91(7):871-81
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory