Gt(ROSA)26Sor^{tm1.1(Aco1*)Mwh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Aco1*)Mwh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Matthias W Hentze
• MGI ID: MGI:5496785
• Synonyms: IRP1*
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Aco1*)Mwh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196374
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(Aco1*)Mwh} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Aco1 (MGI:87879)

• Mutation details: A cDNA encoding Aco1 (Irp1) was mutated by PCR so that 3 cysteines (C437, C503 and C506) were changed to serines and another cysteine (C118) to alanine. These mutations putatively stabilize the apoprotein in the presence of heme. The mutated cDNA (Aco1*) was fused to a FLAG tag and inserted into a plasmid behind a loxP-flanked STOP sequence. The final construct was electroporated into E14 ES cells for homologous recombination. Correctly targeted ES cells were used to generate mice carrying the conditional allele. These animals were crossed to Hprt^tm1(cre)Mnn mice to delete the STOP cassette. (J:196374)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:196374 Casarrubea D, et al., Abnormal body iron distribution and erythropoiesis in a novel mouse model with inducible gain of iron regulatory protein (IRP)-1 function. J Mol Med (Berl). 2013 Jul;91(7):871-81
• All: 1 reference(s)