Gt(ROSA)26Sortm1.1(Aco1*)Mwh
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1.1(Aco1*)Mwh |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Matthias W Hentze |
MGI ID: |
MGI:5496785 |
Synonyms: |
IRP1* |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1.1(Aco1*)Mwh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:196374
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Parent Cell Line: |
E14 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Inserted expressed sequence) |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm1.1(Aco1*)Mwh expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Note |
mouse |
Aco1 (MGI:87879) |
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Mutation details: A cDNA encoding Aco1 (Irp1) was mutated by PCR so that 3 cysteines (C437, C503 and C506) were changed to serines and another cysteine (C118) to alanine. These mutations putatively stabilize the apoprotein in the presence of heme. The mutated cDNA (Aco1*) was fused to a FLAG tag and inserted into a plasmid behind a loxP-flanked STOP sequence. The final construct was electroporated into E14 ES cells for homologous recombination. Correctly targeted ES cells were used to generate mice carrying the conditional allele. These animals were crossed to Hprttm1(cre)Mnn mice to delete the STOP cassette.
(J:196374)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
992 strains or lines available
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Original: |
J:196374 Casarrubea D, et al., Abnormal body iron distribution and erythropoiesis in a novel mouse model with inducible gain of iron regulatory protein (IRP)-1 function. J Mol Med (Berl). 2013 Jul;91(7):871-81 |
All: |
1 reference(s) |
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