Tg(Myh6-cre)TG9Pjay
Transgene Detail

Summary

• Symbol: Tg(Myh6-cre)TG9Pjay
• Name: transgene insertion TG9, Patrick Y Jay
• MGI ID: MGI:5496902
• Synonyms: DCM-TG9, TG 9
• Transgene: Tg(Myh6-cre)TG9Pjay Location: unknown
• Alliance: Tg(Myh6-cre)TG9Pjay page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Myh6-cre)TG9Pjay expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Myh6

• Mutation details: cDNA encoding cre recombinase was cloned into a vector containing a 5.8 kb fragment of the Myh6 (alpha-MHC) promoter including the promoter region and exons 1-3 with the hGH polyadenylation site 3' to the Myh6 fragment. The purified construct was microinjected into male pronuclei of fertilized FVB/N oocytes. 6 founders died with signs of congestive heart failure before germline transmission was established. Two founders transmitted the transgene to produce lines TG 9 and TG 12. TG9 mice developed severe cardiomyopathy and died of heart failure at 11-13 weeks. TG12 animals did not show any pathological cardiac phenotype. The TG9 line was investigated in detail. TG9 cre protein levels are much higher than in TG12 animals, and 50-100 fold higher than in Tg(Myhca-cre)1Abel mice. (J:198043)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myh6 (species not specified) Summary of all recombinase alleles driven by Myh6.

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:198043 Buerger A, et al., Dilated cardiomyopathy resulting from high-level myocardial expression of Cre-recombinase. J Card Fail. 2006 Jun;12(5):392-8
• All: 6 reference(s)