Aloxe3^tm1.2Krie
Targeted Allele Detail

Summary

• Symbol: Aloxe3^tm1.2Krie
• Name: arachidonate lipoxygenase 3; targeted mutation 1.2, Peter Krieg
• MGI ID: MGI:5496904
• Synonyms: eLOX-3^-
• Gene: Aloxe3 Location: Chr11:69016722-69039941 bp, + strand Genetic Position: Chr11, 42.38 cM
• Alliance: Aloxe3^tm1.2Krie page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196489
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxp site was inserted downstream of exon 5. Flp-mediated recombination removed the neomycin resistance cassette and left exons 2 through 5 floxed. Cre-mediated recombination removed exons 2 through 5. Western blot analysis confirmed the absence of protein expression in neonatal mouse epidermis. (J:196489)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aloxe3 Mutation: 34 strains or lines available

References

• Original: J:196489 Krieg P, et al., Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation. J Invest Dermatol. 2013 Jan;133(1):172-80
• All: 1 reference(s)