Tg(Col1a1-FGF2*,-Sapphire)305Mmh
Transgene Detail

Summary

• Symbol: Tg(Col1a1-FGF2*,-Sapphire)305Mmh
• Name: transgene insertion 305, Marja M Hurley
• MGI ID: MGI:5498524
• Synonyms: 18-kDa(305)TgFGF2, Col3.6- LMW-IRES-GFPsaph
• Transgene: Tg(Col1a1-FGF2*,-Sapphire)305Mmh Location: unknown
• Alliance: Tg(Col1a1-FGF2*,-Sapphire)305Mmh page

Trabecular and cortical bone abnormalities in homozygous Tg(Col1a1-FGF2*,-Sapphire)305Mmh and Tg(Col1a1-FGF2*,-Sapphire)308Mmh mice

Show the 3 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Col1a1-FGF2*,-Sapphire)305Mmh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FGF2 (2247)

  Fgf2	(MGI:95516)

• Mutation details: A transgene designed to contain the human low-molecular weight exported 18-kDa isoform of FGF2, an internal ribosome entry site (IRES), and a blue shifted variant of GFP known as Sapphire under the control of a 3.6 kb fragment of the Col1a1 5' regulatory region (Col3.6). The construct also harbors a neomycin selection cassette. Founder line 305 was established. Western blot analysis demonstrates high levels of expression of 18-kDa FGF2 in femurs and calvariae, low levels of expression in liver, and no expression in other tissues. (J:147213)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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tg1	Tg(Col1a1-FGF2*,-Sapphire)305Mmh/Tg(Col1a1-FGF2*,-Sapphire)305Mmh	involves: FVB/N

Phenotypes:

Affected Systems	tg1
show or hide all annotated terms Sex:
growth/size/body	√
increased body weight	√
limbs/digits/tail	√
increased femur compact bone thickness	√
increased femoral compact bone area	√
skeleton	√
abnormal bone marrow morphology	√
increased bone mineral content	√
increased bone mineral density	√
increased bone mineral density of femur	√
increased bone volume	√
abnormal compact bone morphology	√
increased femur compact bone thickness	√
increased femoral compact bone area	√
abnormal periosteum morphology	√
abnormal bone trabecula morphology	√
increased trabecular bone thickness	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:147213 Xiao L, et al., Exported 18-kDa isoform of fibroblast growth factor-2 is a critical determinant of bone mass in mice. J Biol Chem. 2009 Jan 30;284(5):3170-82
• All: 4 reference(s)