Crb1^rd8+em1Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Crb1^rd8+em1Mvw
• Name: crumbs family member 1, photoreceptor morphogenesis associated; endonuclease-mediated revertant 1, Michael Wiles
• MGI ID: MGI:5498686
• Synonyms: corrected, Crb1^cor, Crb1^em1Mvw
• Gene: Crb1 Location: Chr1:139124794-139304838 bp, - strand Genetic Position: Chr1, 60.87 cM
• Alliance: Crb1^rd8+em1Mvw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206789
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NJ

Mutation description

• Allele Type: Endonuclease-mediated
• Mutation: Insertion
• Mutation details: TALEN targeting of exon 9 for homologous recombination with single stranded oligonucleotides (ssODNs) replaces the deleted nucleotide (C/G) in the retinal degeneration 8 (rd8) mutation and incorporates 5 synonymous base substitutions inside the TALEN binding regions. (J:206789)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crb1 Mutation: 92 strains or lines available

References

• Original: J:206789 Low BE, et al., Correction of the Crb1rd8 allele and retinal phenotype in C57BL/6N mice via TALEN-mediated homology-directed repair. Invest Ophthalmol Vis Sci. 2014 Jan;55(1):387-95
• All: 2 reference(s)