Rnf141^tm1Szzh
Targeted Allele Detail

Summary

• Symbol: Rnf141^tm1Szzh
• Name: ring finger protein 141; targeted mutation 1, Sizhong Zhang
• MGI ID: MGI:5499169
• Synonyms: Znf230^ex2-
• Gene: Rnf141 Location: Chr7:110399639-110443664 bp, - strand Genetic Position: Chr7, 57.87 cM, cytoband F2
• Alliance: Rnf141^tm1Szzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198086
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exon 2. RT-PCR confirmed expression of two novel transcripts: a short one splicing exon 1 to 3 and a long one using the neo cassette as a new exon 2. Western blot analysis with antibodies to the C-terminus confirmed expression of a normal sized protein, putatively translated from the long splice variant. (J:198086)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf141 Mutation: 15 strains or lines available

References

• Original: J:198086 Liu Y, et al., A new mutant transcript generated in Znf230 exon 2 knockout mice reveals a potential exon structure in the targeting vector sequence. Acta Biochim Biophys Sin (Shanghai). 2013 Feb;45(2):123-8
• All: 1 reference(s)