Nos3^tm2.1Plh
Targeted Allele Detail

Summary

• Symbol: Nos3^tm2.1Plh
• Name: nitric oxide synthase 3, endothelial cell; targeted mutation 2.1, Paul L Huang
• MGI ID: MGI:5499171
• Synonyms: eNos^S1176A, eNos^SA
• Gene: Nos3 Location: Chr5:24569808-24589472 bp, + strand Genetic Position: Chr5, 11.32 cM
• Alliance: Nos3^tm2.1Plh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198075
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 26 was replaced with a modified one in which nucleotide substitions result in the amino acid substitution of alanine for serine at position 1176 (S1176A). Cre-mediated recombination removed a floxed neomycin resistance cassette. The predicted protein contains an unphosphorylatable (SA) mutation. (J:198075)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nos3 Mutation: 58 strains or lines available

References

• Original: J:198075 Kashiwagi S, et al., eNOS phosphorylation on serine 1176 affects insulin sensitivity and adiposity. Biochem Biophys Res Commun. 2013 Feb 8;431(2):284-90
• All: 6 reference(s)