Kdm6a^tm1.2Afst
Targeted Allele Detail

Summary

• Symbol: Kdm6a^tm1.2Afst
• Name: lysine (K)-specific demethylase 6A; targeted mutation 1.2, A Stewart
• MGI ID: MGI:5499291
• Synonyms: UTX^FD
• Gene: Kdm6a Location: ChrX:18028814-18146175 bp, + strand Genetic Position: ChrX, 13.45 cM
• Alliance: Kdm6a^tm1.2Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196401
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked STOP cassette with a 3' loxP site was inserted upstream of exon 3. A rox flanked hygromycin resistance (hygro) cassette with a 3' loxP site was inserted downstream of exon 3. Flp- and dre-mediated recombination removed the STOP and hygro cassettes. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression. (J:196401)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm6a Mutation: 40 strains or lines available

References

• Original: J:196401 Thieme S, et al., The histone demethylase UTX regulates stem cell migration and hematopoiesis. Blood. 2013 Mar 28;121(13):2462-73
• All: 2 reference(s)