Nxph4^{tm1.1(cre/ERT2)Hze}
Targeted Allele Detail

Summary

• Symbol: Nxph4^{tm1.1(cre/ERT2)Hze}
• Name: neurexophilin 4; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5501233
• Synonyms: Nxph4-2A-CreERT2-D, Nxph4-2A-CreERT2-DeltaNeo
• Gene: Nxph4 Location: Chr10:127361342-127370428 bp, - strand Genetic Position: Chr10, 74.51 cM
• Alliance: Nxph4^{tm1.1(cre/ERT2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199216
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nxph4^{tm1.1(cre/ERT2)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nxph4 (MGI:1336197)

• Mutation details: A targeting vector contained a partial Nxph4 intron sequence containing an frt3 site, a partial Nxph4 exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleavingT2A sequence in-frame with the Nxph4 coding sequence, a cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). (J:199216, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nxph4 (mouse) Summary of all recombinase alleles driven by Nxph4. Comparative matrix view of recombinase activities Alliance mouse Nxph4 gene page

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nxph4 Mutation: 25 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 6 reference(s)