Penk^{tm1.1(cre/ERT2)Hze}
Targeted Allele Detail

Summary

• Symbol: Penk^{tm1.1(cre/ERT2)Hze}
• Name: preproenkephalin; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5501235
• Synonyms: Penk-2A-CreERT2, Penk-2A-CreERT2-DeltaNeo
• Gene: Penk Location: Chr4:4133536-4138815 bp, - strand Genetic Position: Chr4, 2.31 cM
• Alliance: Penk^{tm1.1(cre/ERT2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199217
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Penk^{tm1.1(cre/ERT2)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Penk (MGI:104629)

• Mutation details: A targeting vector contained a partial Penk intron 1 sequence containing an frt3 site, a partial Penk exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleaving T2A sequence in-frame with the Nxph4 coding sequence, a cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, an AttP site and genomic sequence from the Penk locus starting 509 bp downstream of the endogenous stop codon (to create a 511 bp deletion including, the stop codon). This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). (J:199217, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Penk (mouse) Summary of all recombinase alleles driven by Penk. Comparative matrix view of recombinase activities Alliance mouse Penk gene page

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Penk Mutation: 22 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 3 reference(s)