Sec24ctm1c(EUCOMM)Wtsi
Targeted Allele Detail
|
Symbol: |
Sec24ctm1c(EUCOMM)Wtsi |
Name: |
SEC24 homolog C, COPII coat complex component; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5501469 |
Synonyms: |
Sec24cFL |
Gene: |
Sec24c Location: Chr14:20724376-20744920 bp, + strand Genetic Position: Chr14, 11.53 cM
|
Alliance: |
Sec24ctm1c(EUCOMM)Wtsi page
|
IMPC: |
Sec24c gene page |
|
Mutant Cell Line: |
EPD0241_2_A11 |
Germline Transmission: |
Earliest citation of germline transmission:
J:232065
|
Parent Cell Line: |
JM8.N4 (ES Cell)
|
Strain of Origin: |
C57BL/6N
|
Project Collection: |
EUCOMM
|
|
Allele Type: |
|
Targeted (Conditional ready) |
Mutation: |
|
Insertion
Vector: L1L2_Pgk_P
|
|
|
Mutation details: The L1L2_Pgk_P cassette was inserted at position 20679037 of Chromosome 14 upstream of exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 20679926. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, deleting the lacZ seqences and neomycin selection cassette, leaving exon 3 flanked by loxP sites.
(J:232065)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
Cell line EPD0241_2_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232065
|
Original: |
J:232065 Adams EJ, et al., Mammalian COPII coat component SEC24C is required for embryonic development in mice. J Biol Chem. 2014 Jul 25;289(30):20858-70 |
All: |
2 reference(s) |
|