Sec24c^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec24c^{tm1d(EUCOMM)Wtsi}
• Name: SEC24 homolog C, COPII coat complex component; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5501471
• Synonyms: Sec24c^-
• Gene: Sec24c Location: Chr14:20724376-20744920 bp, + strand Genetic Position: Chr14, 11.53 cM
• Alliance: Sec24c^{tm1d(EUCOMM)Wtsi} page
• IMPC: Sec24c gene page

Mutation origin

• Mutant Cell Line: EPD0241_2_A11
• Germline Transmission: Earliest citation of germline transmission: J:232065
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 20679037 of Chromosome 14 upstream of exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 20679926. A null allele was created by flp and cre recombinase expression in mice, resulting in the deletion of the lacZ sequences, the neomycin selection cassette and exon 3. (J:232065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sec24c Mutation: 39 strains or lines available

Notes

Cell line EPD0241_2_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232065

References

• Original: J:232065 Adams EJ, et al., Mammalian COPII coat component SEC24C is required for embryonic development in mice. J Biol Chem. 2014 Jul 25;289(30):20858-70
• All: 1 reference(s)