Rasgrf2^{tm1(cre/folA)Hze}
Targeted Allele Detail

Summary

• Symbol: Rasgrf2^{tm1(cre/folA)Hze}
• Name: RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Hongkui Zeng
• MGI ID: MGI:5501498
• Synonyms: Rasgrf2-2A-dCre, Rasgrf2-2A-DHFR-Cre, Rasgrf2-2A-ecDHFR^R12Y/Y100I/Cre, Rasgrf2-2A-ecDHFRR12Y/Y100I/Cre, Rasgrf2-2A-hDHFR/Cre
• Gene: Rasgrf2 Location: Chr13:92028519-92268164 bp, - strand Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
• Alliance: Rasgrf2^{tm1(cre/folA)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199220
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Rasgrf2^{tm1(cre/folA)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rasgrf2 (MGI:109137)

• Mutation details: The targeting vector containied partial Rasgrf2 intron 29-33 sequence containing an frt3 site in intron 33, a partial Rasgrf2 exon 34 sequence up to (but not including) the endogenous stop codon, self-splicing T2A sequence in-frame with the Rasgrf2 coding sequence, a dCre fusion gene in-frame with the Rasgrf2 coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dCre fusion gene (destabilized Cre, hDHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the E. coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to include the R12Y/Y100I destabilizing domain mutations. The targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. When treated with trimethoprim, cre reporter gene activity is observed at high levels in cortical layers 2/3 and other scattered cells of the cortex, hypothalamus, thalamus, and midbrain. (J:199220, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rasgrf2 (mouse) Summary of all recombinase alleles driven by Rasgrf2. Comparative matrix view of recombinase activities Alliance mouse Rasgrf2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasgrf2 Mutation: 73 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 24 reference(s)