Gt(ROSA)26Sor^tm16Jhai
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm16Jhai
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 16, Jody Haigh
• MGI ID: MGI:5502191
• Synonyms: Gt(ROSA)26Sor^{tm16(rtTA,tetO-Pou5f1,-Sox2,-Klf4,-Myc,-EGFP,-luc)Jhai}, ROSA26-iPSC
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm16Jhai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195147
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting contruct inserted (5' to 3') a wild-type FRT site, splice acceptor site, floxed neomycin resistance/STOP cassette, reverse tetracycline transactivator (rtTA), IRES, puromycin resistance cassette, polyadenylation sequence, tetracycline response element (TRE), a polycistronic Pou5f1, Sox2, Klf4, and Myc transgene, IRES, EGFP, luciferase, polyadenylation sequence, mutated FRT site and promoterless neomycin resistance cassette. (J:195147)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:195147 Haenebalcke L, et al., The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation. Cell Rep. 2013 Feb 21;3(2):335-41
• All: 1 reference(s)