Rasa3tm1b(KOMP)J
Targeted Allele Detail
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Symbol: |
Rasa3tm1b(KOMP)J |
Name: |
RAS p21 protein activator 3; targeted mutation 1b, Jackson |
MGI ID: |
MGI:5502307 |
Gene: |
Rasa3 Location: Chr8:13617218-13727590 bp, - strand Genetic Position: Chr8, 6.29 cM
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Alliance: |
Rasa3tm1b(KOMP)J page
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Mutant Cell Line: |
JKOMP47947-1F2 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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Parent Cell Line: |
JM8A3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Pgk_P
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Mutation details: The HTGRS1006_A_4_H03 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Pgk_P cassette was inserted at position 13615101 of Chromosome 8 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 13614300. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision removed the critical sequence and the neomycin selection cassette yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:148605, J:173534, J:188991)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
5 reference(s) |
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