Clec9a^{tm2.1(icre)Crs}
Targeted Allele Detail

Summary

• Symbol: Clec9a^{tm2.1(icre)Crs}
• Name: C-type lectin domain family 9, member a; targeted mutation 2.1, Caetano Reis e Sousa
• MGI ID: MGI:5502446
• Synonyms: Clec9a^cre
• Gene: Clec9a Location: Chr6:129385825-129401726 bp, + strand Genetic Position: Chr6, 63.3 cM
• Alliance: Clec9a^{tm2.1(icre)Crs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199355
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Clec9a^{tm2.1(icre)Crs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Clec9a (MGI:2444608)

• Mutation details: An existing targeting vector was modified by inserting a codon-improved cre sequence in place of the EGFP sequence in the pFloxRI-C9a-EGFP construct. This construct was electroporated into PRX-B6N (Primogenix) for homologous recombination. Animals carrying the cre allele were crossed to PGK-cre mice to delete the floxed neomycin selection cassette. (J:199355)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Clec9a (mouse) Summary of all recombinase alleles driven by Clec9a. Comparative matrix view of recombinase activities Alliance mouse Clec9a gene page

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Clec9a Mutation: 29 strains or lines available

References

• Original: J:199355 Schraml BU, et al., Genetic Tracing via DNGR-1 Expression History Defines Dendritic Cells as a Hematopoietic Lineage. Cell. 2013 Aug 15;154(4):843-58
• All: 19 reference(s)