Col1a1^{tm1(tetO-CDKN2A)Ibp}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-CDKN2A)Ibp}
• Name: collagen, type I, alpha 1; targeted mutation 1, Ittai Ben-Porath
• MGI ID: MGI:5502558
• Synonyms: tet-p14
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-CDKN2A)Ibp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196969
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-CDKN2A)Ibp} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CDKN2A (1029)

  Cdkn2a

  (MGI:104738)

• Col1a1^{tm1(tetO-CDKN2A)Ibp} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE on KH2 ES cells introduced into the 3' UTR a tetracycline responsive element driving inducible expression of the human cDNA. (J:196969)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cx1	Col1a1^tm1(tetO-CDKN2A)Ibp/Col1a1^+ Tg(KRT5-rtTA)#Glk/0	involves: 129S4/SvJae * C57BL/6 * FVB
cx2	Col1a1^tm1(tetO-CDKN2A)Ibp/Col1a1^tm2(tetO-GFP/RNAi:Trp53)Slowe Tg(KRT5-rtTA)#Glk/0	involves: 129S4/SvJae * C57BL/6 * FVB

Phenotypes:

Affected Systems	cx1	cx2
show or hide all annotated terms
cellular	√	N
cellular phenotype		N
early cellular replicative senescence	√
integument	√	N
integument phenotype		N
alopecia	√
abnormal hair follicle development	√
decreased hair follicle number	√
abnormal hair cycle	√
thick epidermis	√
abnormal skin physiology	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:196969 Tokarsky-Amiel R, et al., Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis. Cancer Res. 2013 May 1;73(9):2829-39
• All: 2 reference(s)