Gt(Ayu21-B137)Imeg^{tm2(CAG-Ptf1aos*/Etn2*)Kymm}
Targeted Allele Detail

Summary

• Symbol: Gt(Ayu21-B137)Imeg^{tm2(CAG-Ptf1aos*/Etn2*)Kymm}
• Name: gene trap Ayu21-B137, Institute of Molecular Embryology and Genetics; targeted mutation 2, Ken-ichi Yamamura
• MGI ID: MGI:5503019
• Synonyms: 21-B137^CAG-mGm13336
• Gene: Gt(Ayu21-B137)Imeg Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: Gt(Ayu21-B137)Imeg^{tm2(CAG-Ptf1aos*/Etn2*)Kymm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195133
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutation: Insertion
• Gt(Ayu21-B137)Imeg^{tm2(CAG-Ptf1aos*/Etn2*)Kymm} expresses 2 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Etn2 (MGI:5502906)
  mouse	Ptf1aos (MGI:3651438)

• Mutation details: Cre-mediated cassette exchange replaces the gene trap cassette with a CAG promoter driving ubiquitous expression of a fusion of exons 1 and 2 of Ptf1aos with 456 bp of the Etn2 sequence with the retrotransposon insertion that produces the Etn2^Sd phenotype (mGm13336) followed by a polyadenylation sequence and FRT-flanked puromycin resistance cassette. (J:195133)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(Ayu21-B137)Imeg Mutation: 1 strain or line available

References

• Original: J:195133 Semba K, et al., Ectopic expression of Ptf1a induces spinal defects, urogenital defects, and anorectal malformations in Danforth's short tail mice. PLoS Genet. 2013 Feb;9(2):e1003204
• All: 1 reference(s)