Nek8^tm1Bei
Targeted Allele Detail

Summary

• Symbol: Nek8^tm1Bei
• Name: NIMA (never in mitosis gene a)-related expressed kinase 8; targeted mutation 1, David R Beier
• MGI ID: MGI:5503122
• Gene: Nek8 Location: Chr11:78056932-78067501 bp, - strand Genetic Position: Chr11, 46.74 cM, cytoband B5
• Alliance: Nek8^tm1Bei page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199559
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing an IRES-lacZ followed by an FRT flanked neomycin selection cassette was inserted in exon 3. No beta-galactosidase activity was detected in embryos. Exon 3 is spliced out of mutant transcripts and splicing of exon 2 to exon 4 causes a frameshift creating a premature stop codon. No protein is detectable by Western blot analysis. (J:199559)

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nek8 Mutation: 28 strains or lines available

References

• Original: J:199559 Manning DK, et al., Loss of the ciliary kinase Nek8 causes left-right asymmetry defects. J Am Soc Nephrol. 2013 Jan;24(1):100-12
• All: 1 reference(s)