Gt(ROSA)26Sor^{tm11(CAG-Braf*/ERT2)Rkuhn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11(CAG-Braf*/ERT2)Rkuhn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Ralf Kuhn
• MGI ID: MGI:5503124
• Synonyms: BRAF^V637EER^T2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11(CAG-Braf*/ERT2)Rkuhn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196873
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm11(CAG-Braf*/ERT2)Rkuhn} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Braf (MGI:88190)

• Mutation details: Recombinase mediated cassette exchange involving Gt(ROSA)26Sor^tm1Rkuhn introduced into the locus a CAG promoter, floxed stop cassette and a fusion of the mouse cDNA containing the amino acid substitution of glutamic acid for valine at position 673 and an N-terminus nuclear export signal to the modified estrogen receptor (ERT2). The mutation is equivalent to the human mutation V600E. (J:196873)

Generation of the Gt(ROSA)26Sor^{tm11(CAG-Braf*/ERT2)Rkuhn} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:196873 Ortiz O, et al., Reversible and tissue-specific activation of MAP kinase signaling by tamoxifen in braf ER mice. Genesis. 2013 Feb 26;:1-8
• All: 1 reference(s)