Tdp2^tm1.1Huy
Targeted Allele Detail

Summary

• Symbol: Tdp2^tm1.1Huy
• Name: tyrosyl-DNA phosphodiesterase 2; targeted mutation 1.1, Danny Huylebroeck
• MGI ID: MGI:5503302
• Synonyms: Tdp2^delta1-3, Tdp2^fldelta
• Gene: Tdp2 Location: Chr13:25015662-25026136 bp, + strand Genetic Position: Chr13, 10.7 cM
• Alliance: Tdp2^tm1.1Huy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195301
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: The first three exons were flanked by loxP sites and an FRT and loxP flanked neomycin resistance cassette was inserted in intron 3. Properly targeted mice were crossed to cre expressing mice to excise exons 1-3 and the selection cassette. No protein activity was seen in protein extracts of cells from deleted mice. (J:195301)

Generation of the Tdp2^tm1Huy and Tdp2^tm1.1Huy alleles

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tdp2^tm1.1Huy

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tdp2 Mutation: 25 strains or lines available

References

• Original: J:195301 Gomez-Herreros F, et al., TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo. PLoS Genet. 2013 Mar;9(3):e1003226
• All: 4 reference(s)