Gabrg2^{tm1.1(Gabrg2)Wwis}
Targeted Allele Detail

Summary

• Symbol: Gabrg2^{tm1.1(Gabrg2)Wwis}
• Name: gamma-aminobutyric acid type A receptor, subunit gamma 2; targeted mutation 1.1, W Wisden
• MGI ID: MGI:5504520
• Synonyms: Gabrg2^tm1.1Wwis, gamma2^I77
• Gene: Gabrg2 Location: Chr11:41801030-41891684 bp, - strand Genetic Position: Chr11, 24.8 cM
• Alliance: Gabrg2^{tm1.1(Gabrg2)Wwis} page

Reduced growth and abnormal posture of Gabrg2^{tm1.1(Gabrg2)Wwis}/Gabrg2^{tm1.1(Gabrg2)Wwis} mice (left)

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199326
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Inserted expressed sequence) Mutation: Insertion Mutation details: A targeting construct inserted in the 5' UTR in exon 1. The vector contains a loxP site, HA-tagged cDNA with (a) nucleotide substitution(s) (and complete with TGA stop codon and polyadenylation sequence), FRT-flanked neomycin resistance cassette and loxP site. The point mutation results in the amino acid substitution of isoleucine for phenylalanine at position 77 (F77I). The predicted protein product is zolpidem-insensitive. Flp-mediated recombination removed the neomycin resistance cassette. In situ hybridization confirmed reduction of transcript expression in the brain. (J:199326)

Generation of the Gabrg2^tm1.1(Gabrg2)Wwis allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabrg2 Mutation: 41 strains or lines available

References

• Original: J:199326 Frola E, et al., Synaptic competition sculpts the development of GABAergic axo-dendritic but not perisomatic synapses. PLoS One. 2013;8(2):e56311
• All: 1 reference(s)