Igh^{tm1.1(b12)Nemz}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm1.1(b12)Nemz}
• Name: immunoglobulin heavy chain complex; targeted mutation 1.1, David Nemazee
• MGI ID: MGI:5505302
• Synonyms: b10H
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205766
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced the DQ52-JH cluster in the Igh locus with HIV antibody b12 variable region coding sequence. Cre-mediated recombination removed the neomycin resistance cassette. (J:205766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:205766 Ota T, et al., B cells from knock-in mice expressing broadly neutralizing HIV antibody b12 carry an innocuous B cell receptor responsive to HIV vaccine candidates. J Immunol. 2013 Sep 15;191(6):3179-85
• All: 2 reference(s)