Igh^{tm2.1(4E10)Nemz}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm2.1(4E10)Nemz}
• Name: immunoglobulin heavy chain complex; targeted mutation 2.1, David Nemazee
• MGI ID: MGI:5505305
• Synonyms: 4E10H
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:205764
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced the DQ52-JH cluster in the Igh gene with HIV antibody 4E10 variable region coding sequence. Cre-mediated recombination removed the neomycin resistance cassette. (J:205764)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:205764 Doyle-Cooper C, et al., Immune tolerance negatively regulates B cells in knock-in mice expressing broadly neutralizing HIV antibody 4E10. J Immunol. 2013 Sep 15;191(6):3186-91
• All: 2 reference(s)