Arhgef12^tm1Sgkd
Targeted Allele Detail

Summary

• Symbol: Arhgef12^tm1Sgkd
• Name: Rho guanine nucleotide exchange factor 12; targeted mutation 1, Silvio Gutkind
• MGI ID: MGI:5505411
• Synonyms: Larg^-
• Gene: Arhgef12 Location: Chr9:42875138-43017069 bp, - strand Genetic Position: Chr9, 24.14 cM, cytoband B
• Alliance: Arhgef12^tm1Sgkd page

Arhgef11^tm1Sgkd/Arhgef11^tm1Sgkd Arhgef12^tm1Sgkd/Arhgef12^tm1Sgkd embryos are less developed with partial branching failure of the cranial vessels

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198274
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A neomycin resistance cassette replaced exon 1. Western blot analysis confirmed the absence of protein expression in the cerebellum. (J:198274)

Generation of the Arhgef12^tm1Sgkd allele

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgef12 Mutation: 89 strains or lines available

References

• Original: J:198274 Mikelis CM, et al., PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. J Biol Chem. 2013 Apr 26;288(17):12232-43
• All: 2 reference(s)