Tg(Retn-cre)1Laz
Transgene Detail

Summary

• Symbol: Tg(Retn-cre)1Laz
• Name: transgene insertion 1, Mitchell A Lazar
• MGI ID: MGI:5505690
• Synonyms: Retn-Cre
• Transgene: Tg(Retn-cre)1Laz Location: unknown
• Alliance: Tg(Retn-cre)1Laz page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Retn-cre)1Laz expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Retn

• Mutation details: A BAC containing the resistin (Retn) gene was modified by inserting a fragmemt encoding a nuclear localization signal (NLS) sequence, cre recombinase, and a polyadenylation signal in front of the start codon of Retn. Start sites of other genes in the BAC were mutated to prevent aberrant transcription. The purified construct was injected into fertilized C57BL/6 oocytes and founders were produced. These were crossed to wild-type and the mice were mated to ROSA26R reporter mice to assess cre activity. The line exhibiting the greatest specificity and efficiency was used for subsequent analysis. (J:199860)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Retn (species not specified) Summary of all recombinase alleles driven by Retn. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:199860 Mullican SE, et al., A novel adipose-specific gene deletion model demonstrates potential pitfalls of existing methods. Mol Endocrinol. 2013 Jan;27(1):127-34
• All: 2 reference(s)