Rorb^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Rorb^{tm1.1(cre)Hze}
• Name: RAR-related orphan receptor beta; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5507855
• Synonyms: Rorb-IRES2-Cre, Rorb-IRES2-Cre-Delta, Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb-IRES2-Cre Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb^{m1(IRES2-Cre)Hze}
• Gene: Rorb Location: Chr19:18907969-19088560 bp, - strand Genetic Position: Chr19, 13.28 cM
• Alliance: Rorb^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Rorb^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rorb (MGI:1343464)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Rorb sequence from intron 9 (with an frt3), a partial Rorb exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Rorb-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove PhiC31 transgene. (J:146821, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rorb (mouse) Summary of all recombinase alleles driven by Rorb. Comparative matrix view of recombinase activities Alliance mouse Rorb gene page

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rorb Mutation: 41 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 28 reference(s)