Col1a1^{tm1(tetO-SOX2)Mjm}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-SOX2)Mjm}
• Name: collagen, type I, alpha 1; targeted mutation 1, Michael J McKay
• MGI ID: MGI:5508552
• Synonyms: TetON-Sox2
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-SOX2)Mjm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199754
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-SOX2)Mjm} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SOX2 (6657)

  Sox2	(MGI:98364)

• Mutation details: RMCE on KH2 ES cells introduced into the 3'UTR a tetracycline responsive element driving inducible expression of a human Sox2 cDNA. (J:199754)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:199754 Kuzmichev AN, et al., Sox2 acts through Sox21 to regulate transcription in pluripotent and differentiated cells. Curr Biol. 2012 Sep 25;22(18):1705-10
• All: 1 reference(s)