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Magi2tm1Key
Targeted Allele Detail
Summary
Symbol: Magi2tm1Key
Name: membrane associated guanylate kinase, WW and PDZ domain containing 2; targeted mutation 1, Charles L Sawyers
MGI ID: MGI:5508897
Synonyms: Magi-2DELTA
Gene: Magi2  Location: Chr5:19432034-20909790 bp, + strand  Genetic Position: Chr5, 8.6 cM, cytoband A3
Alliance: Magi2tm1Key page
Magi2tm1Key/Magi2tm1Key mice develop severe and diffuse glomerular extracapillary proliferations with focal epithelial crescent formation

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:213553
Parent Cell Line:  LW1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA genomic fragment of exon 4 (transcript variant 1) was replaced with a neomycin resistance cassette. Exon 4 encodes a portion of the GUK domain common to all three protein isoforms. Western blot and qRT-PCR analysis performed on brain protein lysates and RNA confirmed the loss of protein and mRNA transcript expression in homozygous mutant mice. (J:213553)
Generation of the Magi2tm1Key allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 8 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Magi2 Mutation:  71 strains or lines available
References
Original:  J:213553 Balbas MD, et al., MAGI-2 scaffold protein is critical for kidney barrier function. Proc Natl Acad Sci U S A. 2014;111(41):14876-81
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory