Magi2^tm1Key
Targeted Allele Detail

Summary

• Symbol: Magi2^tm1Key
• Name: membrane associated guanylate kinase, WW and PDZ domain containing 2; targeted mutation 1, Charles L Sawyers
• MGI ID: MGI:5508897
• Synonyms: Magi-2^DELTA
• Gene: Magi2 Location: Chr5:19432034-20909790 bp, + strand Genetic Position: Chr5, 8.6 cM, cytoband A3
• Alliance: Magi2^tm1Key page

Magi2^tm1Key/Magi2^tm1Key mice develop severe and diffuse glomerular extracapillary proliferations with focal epithelial crescent formation

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213553
• Parent Cell Line: LW1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A genomic fragment of exon 4 (transcript variant 1) was replaced with a neomycin resistance cassette. Exon 4 encodes a portion of the GUK domain common to all three protein isoforms. Western blot and qRT-PCR analysis performed on brain protein lysates and RNA confirmed the loss of protein and mRNA transcript expression in homozygous mutant mice. (J:213553)

Generation of the Magi2^tm1Key allele

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Magi2 Mutation: 72 strains or lines available

References

• Original: J:213553 Balbas MD, et al., MAGI-2 scaffold protein is critical for kidney barrier function. Proc Natl Acad Sci U S A. 2014;111(41):14876-81
• All: 1 reference(s)