Rc3h2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rc3h2^{tm1c(KOMP)Wtsi}
• Name: ring finger and CCCH-type zinc finger domains 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5509140
• Synonyms: Rc3h2^fl
• Gene: Rc3h2 Location: Chr2:37260081-37312915 bp, - strand Genetic Position: Chr2, 24.19 cM
• Alliance: Rc3h2^{tm1c(KOMP)Wtsi} page
• IMPC: Rc3h2 gene page

Mutation origin

• Mutant Cell Line: EPD0131_2_B09
• Germline Transmission: Earliest citation of germline transmission: J:196136
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 37300926 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 37301710. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:196136)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rc3h2 Mutation: 69 strains or lines available

References

• Original: J:196136 Vogel KU, et al., Roquin Paralogs 1 and 2 Redundantly Repress the Icos and Ox40 Costimulator mRNAs and Control Follicular Helper T Cell Differentiation. Immunity. 2013 Apr 18;38(4):655-68
• All: 11 reference(s)