Myf5^{tm1.1(cre/Esr1*)Trdo}
Targeted Allele Detail

Summary

• Symbol: Myf5^{tm1.1(cre/Esr1*)Trdo}
• Name: myogenic factor 5; targeted mutation 1.1, Thomas A Rando
• MGI ID: MGI:5509340
• Synonyms: Myf5^CreER-NN
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^{tm1.1(cre/Esr1*)Trdo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199482
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Myf5^{tm1.1(cre/Esr1*)Trdo} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myf5 (MGI:97252)

• Mutation details: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. To remove the neo marker, mice were bred to B6.129S4-Gt(ROSA)26Sor/RainJ mice. (J:199482)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myf5 (mouse) Summary of all recombinase alleles driven by Myf5. Comparative matrix view of recombinase activities Alliance mouse Myf5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

References

• Original: J:199482 Biressi S, et al., Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Dev Biol. 2013 Jul 15;379(2):195-207
• All: 3 reference(s)