Tpm3^tm3Pgun
Targeted Allele Detail

Summary

• Symbol: Tpm3^tm3Pgun
• Name: tropomyosin 3, gamma; targeted mutation 3, Peter Gunning
• MGI ID: MGI:5509478
• Synonyms: gamma9d-16neo, Tm5/9dneo
• Gene: Tpm3 Location: Chr3:89979958-90008209 bp, + strand Genetic Position: Chr3, 39.2 cM
• Alliance: Tpm3^tm3Pgun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200893
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced most of exon 9d. Western blot analysis confirmed the absence of protein isoforms Tm5NM1 and 2 in the brain or muscle. (J:200893, J:200894)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpm3 Mutation: 48 strains or lines available

References

• Original: J:200893 Vlahovich N, et al., Cytoskeletal tropomyosin Tm5NM1 is required for normal excitation-contraction coupling in skeletal muscle. Mol Biol Cell. 2009 Jan;20(1):400-9
• All: 3 reference(s)