Rnf213^tm1.1Akoi
Targeted Allele Detail

Summary

• Symbol: Rnf213^tm1.1Akoi
• Name: ring finger protein 213; targeted mutation 1.1, Akio Koizumi
• MGI ID: MGI:5510405
• Gene: Rnf213 Location: Chr11:119283926-119378244 bp, + strand Genetic Position: Chr11, 83.48 cM
• Alliance: Rnf213^tm1.1Akoi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:200802
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 20. A neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 20. Cre-mediated recombination removed exon 20 and the neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in the pancreas. (J:200802)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf213 Mutation: 192 strains or lines available

References

• Original: J:200802 Kobayashi H, et al., Ablation of Rnf213 retards progression of diabetes in the Akita mouse. Biochem Biophys Res Commun. 2013 Mar 15;432(3):519-25
• All: 3 reference(s)