Gt(ROSA)26Sor^{tm2(Pik3ca*)Dsa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(Pik3ca*)Dsa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Dieter Saur
• MGI ID: MGI:5510667
• Synonyms: LSL-PIK3CA^H1047R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(Pik3ca*)Dsa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197054
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(Pik3ca*)Dsa} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pik3ca (MGI:1206581)

• Mutation details: The targeting construct inserted into the locus an FRT site, floxed neomycin resistance cassette with 4 polyadenylation sequences, a cDNA encoding a constitutively active form of Pik3ca with an amino acid substitution of arginine for histidine at position 1047 (H1047R), a polyadenyation sequence and an FRT site. Cre-mediated recombination is required to achieve expression. (J:197054)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:197054 Eser S, et al., Selective requirement of PI3K/PDK1 signaling for Kras oncogene-driven pancreatic cell plasticity and cancer. Cancer Cell. 2013 Mar 18;23(3):406-20
• All: 5 reference(s)