Nr6a1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nr6a1^{tm1c(EUCOMM)Wtsi}
• Name: nuclear receptor subfamily 6, group A, member 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5510737
• Synonyms: Gcnf^fl
• Gene: Nr6a1 Location: Chr2:38613381-38816464 bp, - strand Genetic Position: Chr2, 24.42 cM
• Alliance: Nr6a1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Nr6a1 gene page

Mutation origin

• Mutant Cell Lines: EPD0105_6_D03, EPD0105_6_D04
• Germline Transmission: Earliest citation of germline transmission: J:204241
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 38632589 of Chromosome 2 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 38633441. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Flp-mediated recombination removed the reporter and neomycin resistance cassette and left exon 7 floxed. (J:173534, J:204241)

Generation of the Nr6a1^tm1a(EUCOMM)Wtsi, Nr6a1^tm1c(EUCOMM)Wtsi, and Nr6a1^tm1d(EUCOMM)Wtsi alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nr6a1 Mutation: 145 strains or lines available

Notes

Cell line EPD0105_6_D03 was successfully used to make chimeric mice. Germline transmission was accomplished. J:101977

References

• Original: J:173534 Skarnes WC, et al., A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 16;474(7351):337-42
• All: 2 reference(s)