Ensa^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ensa^{tm1a(EUCOMM)Hmgu}
• Name: endosulfine alpha; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5511855
• Gene: Ensa Location: Chr3:95532304-95539413 bp, + strand Genetic Position: Chr3, 40.74 cM
• Alliance: Ensa^{tm1a(EUCOMM)Hmgu} page
• IMPC: Ensa gene page

Mutation origin

• Mutant Cell Lines: HEPD0901_1_A07, HEPD0901_1_A09, HEPD0901_1_A10, HEPD0901_1_B02, HEPD0901_1_B04, HEPD0901_1_B05, HEPD0901_1_B12, HEPD0901_1_C10, HEPD0901_1_D06, HEPD0901_1_D07, HEPD0901_1_D11, HEPD0901_1_D12, HEPD0901_1_E09, HEPD0901_1_E10, HEPD0901_1_F01, HEPD0901_1_F07, HEPD0901_1_F09, HEPD0901_1_G02, HEPD0901_1_G08, HEPD0901_1_G09, HEPD0901_1_H01, HEPD0901_1_H05, HEPD0901_1_H10
• Germline Transmission: Earliest citation of germline transmission: J:271162
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 95533606 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95536162. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Ensa Mutation: 30 strains or lines available

Notes

Cell line HEPD0901_1_A07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:271162

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)