Braf^tm2.2Urr
Targeted Allele Detail

Summary

• Symbol: Braf^tm2.2Urr
• Name: Braf transforming gene; targeted mutation 2.2, Urr R Rapp
• MGI ID: MGI:5512689
• Synonyms: BRaf^del
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm2.2Urr page

Growth retardation in Braf^tm2.2Urr/Braf^tm2.2Urr embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199842
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 3. A floxed neomycin resistance cassette was inserted downstream of exon 3. Cre-mediated recombination removed exon 3 and the neomycin resistance cassette. Western blot analysis confirmed the absence of the two predicted protein isoform expression in embryos. This allele is predicted to produce a truncated, non-functional protein. (J:199842)

Generation of the Braf^tm2Urr, Braf^tm2.1Urr, and Braf^tm2.2Urr alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 60 strains or lines available

References

• Original: J:199842 Pfeiffer V, et al., Ablation of BRaf impairs neuronal differentiation in the postnatal hippocampus and cerebellum. PLoS One. 2013;8(3):e58259
• All: 1 reference(s)