Gt(ROSA)26Sor^tm1.1Ksvo
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1.1Ksvo
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Karel Svoboda
• MGI ID: MGI:5512720
• Synonyms: Rosa26-LSL-H2B-mCherry
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1.1Ksvo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to contain a CAG promoter, followed by a loxP-STOP-LoxP (LSL) sequence and an H2B-mCherry fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Red Fluorescent Protein gene) An AttB-PGKNeo-AttP cassette was placed downstream of the H2B-mCherry fusion protein. This entire construct was inserted into the Gt(ROSA)26Sor locus. PhiC31-mediated recombination removed the neomycin resistance cassette. (J:221246)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:221246 Peron SP, et al., A Cellular Resolution Map of Barrel Cortex Activity during Tactile Behavior. Neuron. 2015 May 6;86(3):783-99
• All: 22 reference(s)