Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh}
Targeted Allele Detail

Summary

• Symbol: Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh}
• Name: paired box 6; targeted mutation 2.1, Xin Zhang
• MGI ID: MGI:5512912
• Synonyms: Pax6^Pax2
• Gene: Pax6 Location: Chr2:105499245-105527709 bp, + strand Genetic Position: Chr2, 55.31 cM
• Alliance: Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh} page

No eye structure is seen in E15.5 homozygous Pax6^{tm2(Pax6_i5a,Pax2)Xzh} or Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh} mutants

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198381
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Pax2

• Mutation details: The coding sequence (exons 4 through 13) was replaced with a loxP site, cDNA encoding an alternatively spliced isoform for Pax6 that contains 14 amino acid inserted within the PD domain that changed its DNA binding specificity (5a), an FRT-flanked neomycin resistance/STOP cassette, loxP site and cDNA encoding Pax2. Cre-mediated recombination removed the Pax6(5a) cDNA and FRT-flanked neomycin resistance/STOP cassette and left the Pax2 cDNA intact. This allele expressed Pax2. (J:198381)

Generation of the Pax6^{tm2(Pax6_i5a,Pax2)Xzh} and Pax6^{tm2.1(Pax6_i5a,Pax2)Xzh} alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax6 Mutation: 94 strains or lines available

References

• Original: J:198381 Carbe C, et al., An allelic series at the paired box gene 6 (Pax6) locus reveals the functional specificity of Pax genes. J Biol Chem. 2013 Apr 26;288(17):12130-41
• All: 1 reference(s)