Tg(Bmx-cre/ERT2)1Rha
Transgene Detail

Summary

• Symbol: Tg(Bmx-cre/ERT2)1Rha
• Name: transgene insertion 1, Ralf H Adams
• MGI ID: MGI:5513853
• Synonyms: Bmx(PAC)-CreERT2
• Transgene: Tg(Bmx-cre/ERT2)1Rha Location: unknown
• Alliance: Tg(Bmx-cre/ERT2)1Rha page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Bmx-cre/ERT2)1Rha expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Bmx (MGI:1101778)

• Mutation details: cDNA encoding cre recombinase fused to the mutated human estrogen receptor ligand-binding domain (ERT2) and followed by a polyadenylation signal and an Frt-flanked neo cassette was inserted into the start codon of the mouse Bmx gene located in the P1 artificial chromosome (PAC; RPCI21) using recombineering methods. Flp-mediated excision in bacteria removed the neo marker. The validated construct was injected into fertilized oocytes to generate founders (details not available). Founders were bred to reporter mice to assess recombination efficiency and specificity; mice display inducible artery-specific cre activity. (J:198633)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Bmx (mouse) Summary of all recombinase alleles driven by Bmx. Comparative matrix view of recombinase activities Alliance mouse Bmx gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:198633 Ehling M, et al., Notch controls retinal blood vessel maturation and quiescence. Development. 2013 Jul;140(14):3051-61
• All: 23 reference(s)