Mterf1a^tm1.2Lrsn
Targeted Allele Detail

Summary

• Symbol: Mterf1a^tm1.2Lrsn
• Name: mitochondrial transcription termination factor 1a; targeted mutation 1.2, Nils-Goran Larsson
• MGI ID: MGI:5514264
• Gene: Mterf1a Location: Chr5:3940581-3943933 bp, - strand Genetic Position: Chr5, 2.26 cM
• Alliance: Mterf1a^tm1.2Lrsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198969
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The second exon of Mterf1a was flanked by a loxP site and an FRT-flanked neomycin resistance cassette with a loxP site. The second exon of Mterf1b was replaced with a hygromycin resistance cassette. ES cells were selected for both mutations on the same chromosome. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed the second exon of Mterf1a. Western blot analysis confirmed the absence of protein expression in the liver. (J:198969)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mterf1a Mutation: 21 strains or lines available

References

• Original: J:198969 Terzioglu M, et al., MTERF1 binds mtDNA to prevent transcriptional interference at the light-strand promoter but is dispensable for rRNA gene transcription regulation. Cell Metab. 2013 Apr 2;17(4):618-26
• All: 1 reference(s)