Kdm1a^tm1.1Sho
Targeted Allele Detail

Summary

• Symbol: Kdm1a^tm1.1Sho
• Name: lysine (K)-specific demethylase 1A; targeted mutation 1.1, Stuart Orkin
• MGI ID: MGI:5514399
• Synonyms: LSD1^fl/fl
• Gene: Kdm1a Location: Chr4:136277851-136330034 bp, - strand Genetic Position: Chr4, 68.8 cM
• Alliance: Kdm1a^tm1.1Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201572
• Parent Cell Line: CJ9 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a single loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 5, and a second loxP site downstream of exon 6. Exons 5 and 6 encode a flavin adenine dinucleotide binding site and the N-terminal portion of the amine oxidase domain, both required for enzymatic activity. FLPe-mediated recombination removed the selection cassette. (J:201572)

Expression

In Mice Carrying this Mutation:	4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kdm1a Mutation: 39 strains or lines available

References

• Original: J:201572 Kerenyi MA, et al., Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. Elife. 2013;2:e00633
• All: 18 reference(s)