Psmb11^{tm1(Psmb8)Khog}
Targeted Allele Detail

Summary

• Symbol: Psmb11^{tm1(Psmb8)Khog}
• Name: proteasome (prosome, macropain) subunit, beta type, 11; targeted mutation 1, Kristin A Hogquist
• MGI ID: MGI:5514401
• Synonyms: beta5t^-
• Gene: Psmb11 Location: Chr14:54862767-54867013 bp, + strand Genetic Position: Chr14, 27.87 cM
• Alliance: Psmb11^{tm1(Psmb8)Khog} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196156
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A cDNA for Psmb8 with a FLAG tag and floxed neomycin resistance cassette replaced the coding sequence. Western blot analysis confirmed the absence of protein expression in the thymus. (J:196156)

Generation of the Psmb11^tm1(Psmb8)Khog and Psmb11^tm1.1(Psmb8)Khog alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmb11 Mutation: 11 strains or lines available

References

• Original: J:196156 Xing Y, et al., Thymoproteasome subunit-beta5T generates peptide-MHC complexes specialized for positive selection. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6979-84
• All: 2 reference(s)