Kl^tm1.2Tel
Targeted Allele Detail

Summary

• Symbol: Kl^tm1.2Tel
• Name: klotho; targeted mutation 1.2, Tobias E Larsson
• MGI ID: MGI:5515329
• Synonyms: beta-KL^-
• Gene: Kl Location: Chr5:150876072-150917282 bp, + strand Genetic Position: Chr5, 89.77 cM, cytoband G3
• Alliance: Kl^tm1.2Tel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201719
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in the kidney. (J:201719)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kl Mutation: 53 strains or lines available

References

• Original: J:201719 Olauson H, et al., Targeted deletion of Klotho in kidney distal tubule disrupts mineral metabolism. J Am Soc Nephrol. 2012 Oct;23(10):1641-51
• All: 4 reference(s)