Ube2m^{tm1.1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ube2m^{tm1.1(KOMP)Wtsi}
• Name: ubiquitin-conjugating enzyme E2M; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5515621
• Synonyms: Ube2m^-
• Gene: Ube2m Location: Chr7:12769047-12772202 bp, - strand Genetic Position: Chr7, 7.73 cM
• Alliance: Ube2m^{tm1.1(KOMP)Wtsi} page
• IMPC: Ube2m gene page

Ube2m^{tm1.1(KOMP)Wtsi}/Ube2m^{tm1.1(KOMP)Wtsi} mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos arrest prior to gastrulation, with mice showing development of the primitive streak but no other hallmarks of gastrulation at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0391_5_A05
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 891 starting at position 12769752 and ending at position 12770643 of Chromosome 7 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site, and Cre-mediated excision deleted this neomycin selection cassette. (J:148605, J:173534, J:188991)

Expression

In Mice Carrying this Mutation:	55 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ube2m Mutation: 21 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 8 reference(s)