Dna2^tm1.1Lzhg
Targeted Allele Detail

Summary

• Symbol: Dna2^tm1.1Lzhg
• Name: DNA replication helicase/nuclease 2; targeted mutation 1.1, Li Zheng
• MGI ID: MGI:5515895
• Gene: Dna2 Location: Chr10:62782805-62809964 bp, + strand Genetic Position: Chr10, 32.51 cM
• Alliance: Dna2^tm1.1Lzhg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198831
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced exons 4 through 7. Cre-mediated recombination removed the neomycin resistance cassette. Western blot analysis confirmed reduced protein expression in heterozygous mouse embryonic fibroblasts. (J:198831)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dna2 Mutation: 61 strains or lines available

References

• Original: J:198831 Lin W, et al., Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity. EMBO J. 2013 May 15;32(10):1425-39
• All: 1 reference(s)