Akap9tm1b(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Akap9tm1b(KOMP)Wtsi |
Name: |
A kinase anchor protein 9; targeted mutation 1b, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5516002 |
Gene: |
Akap9 Location: Chr5:3977410-4130204 bp, + strand Genetic Position: Chr5, 2.26 cM, cytoband A2
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Alliance: |
Akap9tm1b(KOMP)Wtsi page
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IMPC: |
Akap9 gene page |
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Mutant Cell Line: |
EPD0033_5_D02 |
Germline Transmission: |
Earliest citation of germline transmission:
J:198499
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_gt0
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Mutation details: The L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8 at position 4019078. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Cre-mediated recombination removed exon 8 and left the reporter construct intact. Western blot analysis confirmed the absence of protein expression in the pancreas and testis.
(J:198499)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Akap9 Mutation: |
181 strains or lines available
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Cell line EPD0033_5_D02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:198499
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Original: |
J:198499 Schimenti KJ, et al., AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice. Genetics. 2013 Jun;194(2):447-57 |
All: |
1 reference(s) |
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