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Akap9tm1b(KOMP)Wtsi
Targeted Allele Detail
Summary
Symbol: Akap9tm1b(KOMP)Wtsi
Name: A kinase anchor protein 9; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5516002
Gene: Akap9  Location: Chr5:3977410-4130204 bp, + strand  Genetic Position: Chr5, 2.26 cM, cytoband A2
Alliance: Akap9tm1b(KOMP)Wtsi page
IMPC: Akap9 gene page
Mutation
origin
Mutant Cell Line:  EPD0033_5_D02
Germline Transmission:  Earliest citation of germline transmission: J:198499
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 4018123 of Chromosome 5 upstream of the critical exon 8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 8 at position 4019078. The critical exon 8 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Cre-mediated recombination removed exon 8 and left the reporter construct intact. Western blot analysis confirmed the absence of protein expression in the pancreas and testis. (J:198499)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Akap9 Mutation:  181 strains or lines available
Notes
Cell line EPD0033_5_D02 was successfully used to make chimeric mice. Germline transmission was accomplished. J:198499
References
Original:  J:198499 Schimenti KJ, et al., AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice. Genetics. 2013 Jun;194(2):447-57
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory