Kdm6a^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm6a^{tm1b(EUCOMM)Wtsi}
• Name: lysine (K)-specific demethylase 6A; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5516011
• Synonyms: X^UtxGT2Delta
• Gene: Kdm6a Location: ChrX:18028814-18146175 bp, + strand Genetic Position: ChrX, 13.45 cM
• Alliance: Kdm6a^{tm1b(EUCOMM)Wtsi} page
• IMPC: Kdm6a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201868
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 18064967 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 18065789. The critical exon is thus flanked by loxP sites. Subsequent cre expression resulted in a knockout mouse. Deletion of the third exon produces a frameshift and introduction of a translational stop codon when Utx is spliced from exon 2 to exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:201868)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm6a Mutation: 40 strains or lines available

References

• Original: J:201868 Shpargel KB, et al., UTX and UTY demonstrate histone demethylase-independent function in mouse embryonic development. PLoS Genet. 2012 Sep;8(9):e1002964
• All: 4 reference(s)