Gt(ROSA)26Sor^{tm2(CAG-FH*)Pjp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-FH*)Pjp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Patrick J Pollard
• MGI ID: MGI:5518472
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-FH*)Pjp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198486
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, RMCE-ready)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-FH*)Pjp} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FH (2271)

  Fh1	(MGI:95530)

• Mutation details: Recombinase mediated cassette exchange replaced the hygromycin resistance casssette in Gt(ROSA)26Sor^tm1Rkuhn with a neomycin resistance cassette, polyadenylation sequence, FRT site and a C-terminally V5-tagged human cDNA lacking the mitochondrial leader sequence (-MLS; FH^cys) with a polyadenylation sequence driven by a CAG promoter. (J:198486)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

The allele was made in IDG26.10-3 cells that carries Gt(ROSA)26Sor^tm1Rkuhn.

References

• Original: J:198486 Adam J, et al., A role for cytosolic fumarate hydratase in urea cycle metabolism and renal neoplasia. Cell Rep. 2013 May 30;3(5):1440-8
• All: 2 reference(s)